U of T research flips theory on how plaque causes heart disease

The growth of deadly plaque inside the walls of arteries may not happen as scientists believed, new research from the University of Toronto and Massachusetts General Hospital has found.

The research also suggests a new potential target in the treatment of atherosclerosis, a leading cause of cardiovascular disease and death globally.

The research team found that macrophages, white blood cells that drive atherosclerosis, replicate inside plaques. Moreover, this growth is not reliant on cells outside the plaques called monocytes, as scientists had assumed.

“Until now, the thinking was that inflammatory macrophages arise mainly from the recruitment of their precursors — monocytes — from the bloodstream,” said Clint Robbins, lead author on the study and an Assistant Professor in U of T’s Departments of Laboratory Medicine and Pathobiology, and Immunology. “Our study shows that the accumulation of macrophages also depends on their proliferation locally within the developing plaque.”

The journal Nature Medicine published the study results on August 11.

The impact of the research on clinical treatments could be large. Many pharmaceutical companies are pouring resources into potential therapies that can block the recruitment of white blood cells into plaques. But if macrophages self-sustain through local cell division, blocking recruitment may not be the best strategy.

"I think this work will force some major re-evaluations," said Filip Swirski, the study’s principal investigator who is a scientist in the Center for Systems Biology at Massachusetts General Hospital and an Assistant Professor at Harvard Medical School. "People have been thinking of targeting monocyte influx to treat atherosclerosis, but they need to consider macrophage proliferation as an additional or alternative approach, especially in established disease.”

That approach might be better than targeting circulating monocytes, since interrupting disease-causing processes within plaques could spare other beneficial immune responses that monocytes control, said Swirski. 

(Image below: White blood cells called macrophages replicate in growing plaques, contribute to atherosclerosis.)

As well, it could help improve the current standard of care in treating atherosclerosis: statin therapy. Statins, in addition to lowering blood lipids that contribute to plaque, have anti-inflammatory properties. The researchers are now looking at whether statins might limit the spread of macrophages within plaques.

“Additional targeting of macrophage proliferation may further decrease inflammation in atherosclerosis and prove clinically advantageous,” said Robbins, who is also a scientist in the Toronto General Research Institute at University Health Network.

The researchers conducted their study in mice, and they caution that much more research is needed to see how the work will translate to humans. But encouragingly, they found evidence of macrophage growth in plaques from human carotid arteries.

Next, the team will compare macrophage proliferation to monocyte recruitment during different stages of atherosclerosis, and look at whether all macrophages, or only subsets, replicate.

The study was funded by the U.S. National Institutes of Health, the Massachusetts General Hospital, the Heart and Stroke Richard Lewar Centre of Excellence in Cardiovascular Research, and the Department of Laboratory Medicine and Pathobiology at the University of Toronto.

Jim Oldfield is a writer with the Faculty of Medicine at U of T; this article was partly adapted from a Massachusetts General Hospital story by Sue McGreevey.